Evaluation of Effect of Plasmas Prepared from Packed Red Blood Cells at Different Weeks on Production of Nitric Oxide (NO) and Malondialdehyde (MDA) from Peripheral Blood Mononuclear Cells in Vitro

Introduction: In present study, we evaluated the effect of plasma collected from packed red blood cells (PRBCs) at various weeks after donation on peripheral blood mononuclear cells (PBMCs). Methods: In this experimental study, plasma prepared from twenty PRBCs in the first, second, fourth, and fifth weeks after donation. PBMCs were also isolated from healthy donors and treated with different concentrations of prepared plasma in vitro. NO and MDA levels were measured in culture supernatant using Griess reaction and thiobarbituric acid method, respectively and cell activity was evaluated using MTT assay. GraphPad Prism (version 5) software was used for statistical analysis (repeated measure) of the data. P values less than 0.05 were considered statistically significant. Results: Our results indicated that plasma prepared from PRBCs during the early weeks increases the activity of PBMCs and enhance NO production. We also found that these plasmas have various effects on concentration of MDA released from PBMCs depending on time and concentration. Conclusions: We concluded that plasma of PRBCs can have immunostimulatory or immunosuppressive effects on PBMCs, depending on the concentration and duration of blood bag storage.